2013年9月 ~ 至今： 中國科學院上海有機化學研究所 研究員、課題組長
2008年6月 ~ 2013年8月： 美國霍華德休斯醫(yī)學研究院 博士后/助理研究員
2008年1月 ~ 2008年5月： 美國賓夕法尼亞大學 博士后
2002年9月?~ 2007年12月：美國賓夕法尼亞大學 神經(jīng)科學博士學位
1997年9月?~ 2002年7月: ?北京大學醫(yī)學部 醫(yī)學學士學位?
神經(jīng)軸突退化變性壞死是急性神經(jīng)損傷如脊髓損傷和腦創(chuàng)傷以及很多與衰老相關的慢性神經(jīng)退行性疾病如老年癡呆癥(AD)、帕金森氏癥(PD)、亨廷頓氏癥(HD)、多發(fā)性硬化癥(MS)、肌萎縮性脊髓側(cè)索硬化癥(ALS)等疾病中的常見病變。此外，糖尿病、青光眼和化療引起的神經(jīng)毒性也會導致繼發(fā)性的神經(jīng)軸突的退化變性壞死。由于人類的中樞神經(jīng)在受損或變性壞死后再生能力極其有限，如何保護受損或患病的神經(jīng)，預防、延緩、阻止乃至逆轉(zhuǎn)神經(jīng)軸突退化變性壞死是生物醫(yī)學領域的一個重大前沿課題。
方燕姍課題組致力于急性神經(jīng)損傷和慢性退行性疾病中神經(jīng)退化的細胞和分子機理的研究，運用全新的神經(jīng)損傷活體動物模型結合分子遺傳學、細胞生物學、生物化學、藥物化學、超高分辨率熒光成像、新一代測序技術、現(xiàn)代蛋白質(zhì)組學和代謝組學等技術研究調(diào)控神經(jīng)退化和促進神經(jīng)再生修復的分子機制，并以此作為基礎開發(fā)用于疾病診斷治療的生物標志物和新型藥物。
2023年?獲得國家杰出青年科學基金
2023年?入選首批上海浦東“明珠計劃”領軍人才
2014年 入選國家高技術研究發(fā)展計劃（863計劃）青年科學家
2014年 入選國家高層次人才計劃青年項目
1.?Shu S, Jiang M, Deng X, Yue W, Cao X, Zhang K, Wang Z, He H, Cui J, Wang Q, Qu K and FANG Y#. (2023) “Heterochromatic silencing of immune-related genes in glia is required for BBB integrity and normal lifespan in Drosophila.” Aging Cell, 22:e13947.
2.?Yue W, Zhang K, Jiang M, Long W, Cui J, Li Y, Zhang Y, Li A#?and FANG Y#. (2023) “Deubiquitination of SARM1 by USP13 regulates SARM1 activation and axon degeneration”. Life Medicine, 2(5):1-12.
3.?Yue W, Tang CW and FANG Y#. (2023) “PIKFYVEinhibition, a neuronal “emetic” for treating ALS?” Neuroscience Bulletin, 39(11):1738-1740.
4.?Yue W, Deng X, Wang Z, Jiang M, Hu R, Duan Y, Wang Q, Cui J and FANG Y#. (2023) “Inhibition of the MEK/ERK pathway suppresses immune overactivation and mitigates TDP-43 toxicity in a Drosophila?model of ALS.” Immunity & Ageing,?20(1):27.
5.?Ni J, Ren Y, Su T, Zhou J, Fu C, Yi Lu, Li D, Zhao J, Li Y, Zhang Y, FANG Y, Liu N, Geng Y#?and Chen Y#. (2023) “Loss of TDP-43 function underlies hippocampal and cortical synaptic deficits in TDP-43 proteinopathies.” Molecular Psychiatry?28(2):?931-945.
6.?Hu R, Qian B, Li A#?and FANG Y#. (2022) “Role of Proteostasis Regulation in the Turnover of Stress Granules.” Int J Mol Sci, 23(23):14565.
7.?Deng X, Sun X, Yue W, Duan Y, Hu R, Zhang K, Ni J, Cui J, Wang Q, Chen Y, Li A#?and FANG Y#. (2022) “CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.” Journal of Cell Biology, 221(1):?e202103033.
8.?Li Y, Gu J, Wang C, Hu J, Zhang S, Liu C, Zhang S,?FANG Y?and Li D#. (2022) “Hsp70 exhibits a liquid-liquid phase separation ability and chaperones condensed FUS against amyloid aggregation.”?iScience. 25(6):104356.
9.?Huang C, Lu J, Ma X, Qiang J, Wang C, Liu C,?FANG Y, Zhang Y, Jiang L, Li D#?and Zhang S#. (2022) “The mouse nicotinamide mononucleotide adenylyltransferase chaperones diverse pathological amyloid client proteins.”?Journal of Biological Chemistry,?298(5):101912.
10.?Zhang K, Jiang M and FANG Y#. (2021) “The drama of Wallerian degeneration: the cast, crew and script.” Annual Review of Genetics, 55:93-113.
11.?Zhang?K, Wang Q, Liang Y, Yan Y, Wang H, Cao X, Shan B, Zhang Y#, Li A#?and FANG Y#. (2021) “Quantitative proteomic analysis of mouse sciatic nerve reveals post-injury upregulation of ADPGK promoting macrophage phagocytosis.” Frontier in Molecular Neuroscience, 14:777621.
12.?Gu J, Wang C, Hu R, Li Y, Zhang S, Sun Y, Wang Q, Li D, FANG Y#?and Liu C#. (2021) “Hsp70 chaperones TDP-43 in dynamic, liquid-like phase and prevents it from amyloid aggregation.” Cell Research, 31(9):1024-1027.
13.?Wang H, Zhang Y, Zeng K, Qiang J, Cao Y, Li Y, FANG Y, Zhang Y#?and Chen Y#. (2021) “Selective mitochondrial protein labeling enabled by biocompatible photocatalytic reactions inside live cells.” JACS Au, 1(7):1066-1074.
14.?Sun Y, Zhao K, Xia W, Feng G, Gu J, Ma Y, Gui X, Zhang X, FANG Y, Sun B, Wang R, Liu C#?and Li D#. (2020) “The nuclear localization sequence mediates hnRNPA1 amyloid fibril formation revealed by cryoEM structure.” Nature Communications, 11(1):6349.
14.?Gu J, Liu Z, Zhang S, Li Y, Xia W, Wang C, Xiang H, Liu Z, Tan L, FANG Y, Liu C#?and Li D#. (2020) “Hsp40 proteins phase separate to chaperone the assembly and maintenance of membraneless organelles.” Proc Natl Acad Sci U S A, 117(49):31123-31133.
15.?Wang C, Duan Y, Duan G, Wang Q, Zhang K, Deng X, Qian B, Gu J, Ma Z, Zhang S, Guo L, Liu C#?and?FANG Y#. (2020) “Stress induces dynamic, cytotoxicity-antagonizing TDP-43 nuclear bodies via paraspeckle lncRNA NEAT1-mediated liquid-liquid phase separation.” Molecular Cell, 79(3):443-458.e7. (Highlighted with commentary by Malik and Barmada in Molecular Cell)
16.?Ma X, Zhu Y, Lu J, Xie J, Li C, Shin WS, Qiang J, Liu J, Dou S, Xiao Y, Wang C, Jia C,?Long H, Yang J, FANG Y, Jiang L, Zhang Y, Zhang S, Zhai RG#, Liu C#?and Li?D#. (2020) “Nicotinamide mononucleotide adenylyltransferase uses its NAD(+)?substrate-binding site to chaperone phosphorylated Tau.” Elife, 9. pii:e51859.
17.?Liu C#?and FANG Y#. (2019) “New insights of poly(ADP-ribosylation) in neurodegenerative diseases: a focus on protein phase separation and pathologic aggregation.” Biochemical Pharmacology, 167:58-63.
18.?Duan Y, Du A, Gu J, Duan G, Wang C, Gui X, Ma Z, Qian B, Deng X, Zhang K, Sun L, Tian K, Zhang Y, Jiang H, Liu C#?and FANG Y#. (2019) “PARylation regulates stress granule dynamics, phase separation, and neurotoxicity of disease-related RNA-binding proteins.” Cell Research, 29(3):233-247.
19.?Wang H, Wang X, Zhang K, Wang Q, Cao X, Wang Z, Zhang S, Li A#?Liu K#?and FANG Y#. (2019) “Rapid depletion of ESCRT protein Vps4 underlies injury-induced autophagic impediment and Wallerian degeneration.” Science Advances, 5(2):eaav4971.
20.?Yang L, Cao Y, Zhao J, FANG Y, Liu N#?and Zhang Y#. (2019) “Multidimensional proteomics identifies declines in protein homeostasis and mitochondria as early signals for normal aging and age-associated disease in Drosophila.” Mol Cell Proteomics, 8(10):2078-2088.
21.?Sun X, Duan Y, Qin C, Li JC, Duan G, Deng X, Ni J, Cao X, Xiang K, Tian K, Chen CH, Li A#?and FANG Y#.?(2018) “Distinct multilevel misregulations of Parkin and PINK1 revealed in cell and animal models of TDP-43 proteinopathy.” Cell Death & Disease, 9(10):953.
22.?Cao X, Wang H, Wang Z, Wang Q, Zhang S, Deng Y and FANG Y#. (2017) “In vivo?imaging reveals mitophagy independence in the maintenance of axonal mitochondria during normal aging.”?Aging Cell, 16(5):1180-1190.
23.?Cao X and FANG Y#. (2015) “Transducing oxidative stress to death signals in neurons.” Journal of Cell Biology, 211(4):741-743.
24.?FANG Y#?and Boinini NM#. (2015) “Hope on the fruit fly - The Drosophila?wing paradigm of axon injury.” Neural Regeneration Research, 10(2):173-175.
25.?Huang Q, Yang L, Luo J, Guo L, Wang Z, Yang X, Jin W, FANG Y, Ye J, Shan B#?and Zhang Y#. (2015) “SWATH enables precise label-free quantification on proteome scale.” Proteomics, 15(7):1215-23.
26.?FANG Y, Soares L and Bonini NM#. (2013) “Design and implementation of in vivo?imaging of neural injury responses in the adult Drosophila?wing.”?Nature Protocols, 8(4): 810-819.?
27.?Garbe DS, FANG Y, Zheng X, Sowcik M, Anjum R, Gygi SP and Sehgal A#. (2013) “Cooperative interaction between phosphorylation sites on PERIOD maintains circadian period in Drosophila.” PLoS Genetics,?9(9): e1003749.
28.?FANG Y?and Boinini NM#. (2012) “Axon degeneration and regeneration: insights from Drosophila?models of nerve injury.” Annual Review of Cell and Developmental Biology,?28:575-597.
29.?FANG Y, Soares L, Teng X, Geary M and Bonini NM#. (2012) “A novel Drosophila?model of nerve injury reveals an essential role of Nmnat in maintaining axonal integrity.” Current Biology, 22(7):590-595. (Highlighted with commentary by Wang and Barres in Current Biology; Recommended by Faculty of 1000)
30.?FANG Y?and Sehgal A#. (2010) “Protein phosphatases and circadian clocks.” in Handbook of Cell Signaling, 2nd Edition,?eds. Bradshaw, R. A. & Dennis, E. A. Oxford:Academic Press, pp. 877-882.?
31.?Wu Y, Bolduc FV, Bell K, Tully T, FANG Y, Sehgal A and Fischer JA#. (2008) “A Drosophila?model for Angelman syndrome.” Proc Natl Acad Sci U S A, 105(34):12399-12404.
32.?FANG Y, Sathyanarayanan S and Sehgal A#. (2007) “Post-translational regulation of the Drosophila?circadian clock requires protein phosphatase 1 (PP1).” Genes & Development, 21(12):1506-1518.
33.?Sehgal A, Joiner W, Crocker A, Koh K, Sathyanarayanan S, FANG Y, Wu M, Williams J?and Zheng X#. (2007) “Molecular analysis of sleep:wake cycles in Drosophila.”?Cold Spring Harb Symp Quant Biol, 72:557-564.
34.?Stein JM, Bergman W, FANG Y, Davison L, Brensinger C, Robinson MB, Hecht NB and Abel T#. (2006) “Behavioral and neurochemical alterations in mice lacking the RNA-binding protein Translin.” Journal of Neuroscience, 26(8):2184-2196.
